Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

In vivo and in vitro effects of Bletilla striata polysaccharide-loaded paclitaxel nanoparticles on human gastric cancer cells

Li Xuchen, Bai Guang

General Surgery, The First Affiliated Hospital of Jinzhou Medical University, No 2, Section 5, Renmin Street, Guta District, Jinzhou City, Liaoning Province 121000, China;

For correspondence:- Bai Guang Email: pr1181@163.com

Accepted: 27 December 2018 Published: 31 January 2019

Citation: Xuchen L, Guang B. In vivo and in vitro effects of Bletilla striata polysaccharide-loaded paclitaxel nanoparticles on human gastric cancer cells. Trop J Pharm Res 2019; 18(1):13-17 doi: 10.4314/tjpr.v18i1.2

© 2019 The authors.
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Abstract

Purpose: To investigate the in vivo and in vitro effects of paclitaxel nanoparticle (PTX)-loaded Bletilla striata polysaccharide (BSP) on human gastric cancer cells.

Methods: Mice weighing 13 to 17 g and aged 4 to 6 weeks, were inoculated with human gastric gland cancer cell line (MKN45), and randomly assigned to five groups: control group, PTX-1 (10 mk/kg) group; PTX-2 (15 mg/kg) group, BSP-PTX-1 (10 mg/kg) group, and BSP-PTX-2 (15 mg/kg) group. The anti-proliferative influence of BSP-PTX and its cellular target were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and fluorescence microscopy, respectively.

Results: Inhibition of MKN45 cells was significantly higher in BSP-PTX group (88.24 %) than in PTX group (76.74 %, p < 0.05). More BSP-PTX entered the cells than PTX. Tumor inhibition was significantly low in PTX-1 group (37.58 %), relative to the BSP-PTX-I group (45.00 %, p < 0.5). In addition, tumor inhibition was significantly lower in PTX-2 group (52.35 %) than in BSP-PTX-2 group (69.80 %, p < 0.5). The weight gain of mice was lower in the PTX or BSP-PTX groups than in control mice, while the weight gain of mice in BSP-PTX-2 group (26.35 %) was significantly higher than that of PTX-2 group (19.43 %, p < 0.5).

Conclusion: Bletilla striata polysaccharide-loaded paclitaxel nanoparticles enhance drug delivery, and effectively and safely exert anti-proliferative effect on MKN45 cells and in mice. Thus, these nanoparticles have good potential for development into anti-gastric cancer agents for clinical application

Keywords: Bletilla striata polysaccharide, Paclitaxel nanoparticles, Human gastric cancer cells, Tumor target, Liver cancer